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Reverse-Phase Chromatography

Meaning

Reverse-Phase Chromatography (RPC) is a high-precision analytical technique utilized in clinical chemistry and pharmaceutical quality control to separate, identify, and quantify components in a complex mixture, such as biological fluids or synthetic peptide batches. This method relies on the differential partitioning of molecules between a non-polar stationary phase and a polar mobile phase, effectively separating compounds based on their hydrophobicity. In the hormonal space, RPC is essential for isolating and purifying peptides and steroid metabolites, ensuring the accuracy and purity of both diagnostic assays and therapeutic agents.