Question 1

What is the Meaning of Recombinant DNA Technology?

Accepted Answer

Recombinant DNA Technology involves the precise manipulation of genetic material to combine DNA sequences from different biological sources, creating novel genetic constructs that can be introduced into host cells for specific purposes.

Question 2

What is the Context of Recombinant DNA Technology?

Accepted Answer

This molecular engineering approach is fundamental to modern biotechnology and the pharmaceutical industry, forming the basis for producing a wide array of therapeutic proteins, including those critical for hormonal regulation and metabolic balance within the human physiological system.

Question 3

What is the Significance of Recombinant DNA Technology?

Accepted Answer

Its clinical importance lies in enabling the large-scale, consistent production of human proteins like insulin and growth hormone, which were once scarce, thereby providing life-saving treatments for conditions such as diabetes and growth hormone deficiency, directly improving patient health outcomes and quality of life.

Question 4

What is the Mechanism of Recombinant DNA Technology?

Accepted Answer

The process typically begins with isolating a specific gene of interest, often encoding a therapeutic protein; this gene is then inserted into a vector, commonly a bacterial plasmid, which serves as a vehicle to carry the gene into a suitable host cell where it is replicated and expressed to yield the desired protein product through the host's cellular machinery.

Question 5

What is the Application of Recombinant DNA Technology?

Accepted Answer

Clinically, this technology is widely applied in the manufacture of recombinant human insulin for diabetes management, recombinant human growth hormone for pediatric and adult deficiencies, and various other biopharmaceutical agents, including therapeutic antibodies used in autoimmune conditions and cancer therapies, along with advancements in gene editing tools.

Question 6

What is the Metric of Recombinant DNA Technology?

Accepted Answer

The efficacy of recombinant protein therapies is routinely monitored by assessing clinical symptom resolution, measuring the patient's physiological response to treatment, and quantifying the levels of the administered therapeutic protein or its downstream effects through specific blood tests or biomarker assays relevant to the treated condition.

Question 7

What is the Risk of Recombinant DNA Technology?

Accepted Answer

While generally well-tolerated and rigorously regulated, potential risks associated with recombinant DNA-derived therapeutics include the possibility of an immune response against the foreign protein, leading to reduced efficacy or adverse reactions, and the theoretical risk of unintended genetic alterations in certain gene therapy applications, necessitating careful patient selection and ongoing vigilance.

Recombinant DNA Technology

Meaning

Context

Significance

Mechanism

Application

Metric

Risk

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