What is the Origin of PvuII and XbaI?

The names PvuII and XbaI are scientific nomenclature derived from the bacteria from which they were isolated: Proteus vulgaris and Xanthomonas badrii, respectively. The Roman numerals indicate the order of discovery. Their application in human genetics, specifically for analyzing steroid receptor genes, was a key technique in early molecular endocrinology.

What is the Mechanism of PvuII and XbaI?

PvuII recognizes the sequence CAGCTG, and XbaI recognizes TCTAGA. If a specific single nucleotide polymorphism (SNP) is present within the ERα gene sequence, it can either create or abolish one of these restriction sites. When genomic DNA is incubated with the enzyme, the presence or absence of the cleavage site results in DNA fragments of different lengths. Analyzing these fragment lengths via gel electrophoresis allows for the genotyping of the individual's

PvuII and XbaI ∞ Area

PvuII and XbaI

Meaning

PvuII and XbaI are two specific restriction endonucleases, which are enzymes derived from bacteria that recognize and cleave DNA at precise, distinct recognition sequences. In the context of hormonal health, these enzymes are clinically relevant because they were historically used to identify specific polymorphisms, or variations, within the human Estrogen Receptor alpha (ERα) gene. These variations, often detected as Restriction Fragment Length Polymorphisms (RFLPs), are associated with differential responsiveness to estrogen and risk for hormone-related conditions.