What is the Origin of Mitochondrial Function Assay?

The development of these assays is rooted in cellular bioenergetics and biochemistry, evolving from early respirometry techniques to sophisticated high-throughput instruments like the Seahorse Analyzer. The clinical relevance of mitochondrial function has expanded significantly in recent decades, moving from basic research to becoming a key diagnostic tool in metabolic and longevity medicine.

What is the Mechanism of Mitochondrial Function Assay?

The assay typically involves subjecting living cells to various metabolic substrates and inhibitors while measuring their real-time oxygen consumption and proton efflux. The oxygen consumption rate directly reflects the activity of the electron transport chain, quantifying oxidative phosphorylation. Extracellular acidification measures glycolysis. Analyzing these metabolic rates provides a comprehensive profile of both aerobic and anaerobic energy production pathways.

Mitochondrial Function Assay ∞ Area

Mitochondrial Function Assay

Meaning

A Mitochondrial Function Assay is a specialized laboratory test designed to quantitatively assess the health and efficiency of a cell’s mitochondria, the organelles responsible for producing the vast majority of cellular energy (ATP). These assays measure parameters such as oxygen consumption rate, extracellular acidification rate, and ATP production capacity. The data provides crucial insight into cellular metabolic health, which is directly linked to vitality and the aging process.