Microbial community sequencing is a high-throughput molecular diagnostic technique used to determine the precise genetic identity and relative abundance of the entire collection of microorganisms, or microbiome, present in a biological sample, such as stool. This technology involves sequencing marker genes, like the 16S rRNA gene, or the entire microbial DNA (shotgun metagenomics) to provide a detailed, functional blueprint of the gut ecosystem. Clinicians utilize this comprehensive data to move beyond simple pathology and understand the functional capacity of the microbial community in relation to host health and endocrine function.
Origin
This methodology is a direct product of advances in molecular biology and next-generation DNA sequencing technology. The application of these techniques to complex microbial samples allowed for the first time an accurate, culture-independent assessment of microbial diversity and composition. This development launched the modern era of microbiome research and its clinical application.
Mechanism
The sequencing mechanism relies on extracting all DNA from the sample, amplifying specific genetic regions common to bacteria and other microbes, and then reading the nucleotide sequence of those amplified fragments. Bioinformatics tools compare these sequences against vast reference databases to identify the species present and their relative proportions. This data allows for the calculation of diversity indices and the prediction of metabolic functions, providing actionable insights into the gut-hormone axis.
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