What is the Origin of LC-MS/MS Assay?

The term is an acronym representing the combined analytical instruments: Liquid Chromatography (LC) and tandem Mass Spectrometry (MS/MS). The technique evolved from earlier forms of mass spectrometry, integrating the separation power of chromatography with the identification power of mass analysis. Its widespread adoption in the clinical laboratory is a testament to the need for high-fidelity measurements in modern endocrinology.

What is the Mechanism of LC-MS/MS Assay?

Liquid Chromatography first separates the various components of the sample based on their chemical properties, such as polarity, as they pass through a column. The separated compounds then enter the tandem Mass Spectrometer, where they are ionized and fragmented in a controlled manner. The first mass analyzer selects the specific ion of interest (the precursor ion), and the second mass analyzer measures the mass of its unique fragments (the product ions), confirming the molecule's identity and quantifying its concentration based on the intensity of the signal.

LC-MS/MS Assay

Meaning

The LC-MS/MS assay is a highly sophisticated and precise analytical technique combining Liquid Chromatography (LC) with tandem Mass Spectrometry (MS/MS) to separate, identify, and quantify specific compounds, such as hormones or metabolites, within a complex biological sample. This methodology is recognized as the gold standard in clinical endocrinology for the accurate measurement of low-concentration steroid hormones due to its superior sensitivity, specificity, and ability to differentiate between structurally similar molecules. It provides clinicians with robust, unambiguous data for precise diagnosis and therapeutic monitoring.

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LC-MS/MS Assay

Meaning

Origin

Mechanism

What Are the Specific Monitoring Protocols for Women on Testosterone Therapy?

What Are the Clinical Guidelines for Prescribing Peptide Hormonal Support?

What Monitoring Protocols Are Essential for Sustained Female Testosterone Optimization?

What Are the Specific Monitoring Requirements for Women Undergoing Testosterone Optimization?

What Specific Laboratory Markers Are Essential for Monitoring Testosterone Therapy Outcomes?

What Are the Diagnostic Criteria for Initiating Testosterone Replacement Therapy in Women?

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