What is the Origin of Hormone Assays?

The term combines 'hormone,' from the Greek 'hormao,' meaning to set in motion, with 'assay,' an old French term meaning to try or test. Early hormone assays relied on bioassays, but modern endocrinology utilizes highly specific methods like radioimmunoassays (RIA), enzyme-linked immunosorbent assays (ELISA), and mass spectrometry (LC-MS/MS). The evolution of these techniques has enabled the detection of hormones at increasingly minute, physiologically relevant concentrations.

What is the Mechanism of Hormone Assays?

Most modern hormone assays operate on the principle of competitive or non-competitive binding, where the target hormone in the sample competes with a labeled version of the hormone for binding sites on a specific antibody. In mass spectrometry, the mechanism involves separating hormones based on their mass-to-charge ratio after ionization, offering superior specificity and accuracy, particularly for low-concentration steroids. The resulting signal is proportional to the hormone concentration, providing the clinician with a precise numerical value for diagnostic interpretation.

Hormone Assays

Meaning

Hormone assays are laboratory techniques utilized to quantitatively measure the concentration of specific hormones within biological fluids, such as blood, saliva, or urine. These quantitative assessments provide clinicians with objective data to diagnose endocrine disorders, monitor the efficacy of hormonal therapies, and evaluate the overall functional status of the endocrine system. The precision and sensitivity of these assays are paramount for accurate clinical decision-making and personalized treatment titration.

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Hormone Assays

Meaning

Origin

Mechanism

The Hormonal Code to Effortless Weight Control

Beyond Aging: Designing Your Future Physiology

The Next-Level Vitality Equation

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