Question 1

What is the Meaning of GC-MS/MS?

Accepted Answer

Gas Chromatography-Tandem Mass Spectrometry, GC-MS/MS, is an advanced analytical technique. It combines gas chromatography's separation with tandem mass spectrometry's precise detection. This method identifies and quantifies specific chemical compounds, even at low concentrations, within complex biological samples, offering high sensitivity and selectivity.

Question 2

What is the Context of GC-MS/MS?

Accepted Answer

In hormonal health, GC-MS/MS analyzes steroid hormones, their metabolites, and other biomolecules. This technology operates within specialized clinical and research laboratories. Its application provides insights into metabolic pathways and endocrine function, aiding understanding of human physiology and supporting diagnostic efforts.

Question 3

What is the Significance of GC-MS/MS?

Accepted Answer

GC-MS/MS holds substantial clinical importance, providing definitive identification and precise hormone quantification. It is essential for diagnosing endocrine disorders like congenital adrenal hyperplasia and Cushing's syndrome. The method offers superior accuracy over immunoassays, especially for low-concentration analytes or where cross-reactivity is a concern.

Question 4

What is the Mechanism of GC-MS/MS?

Accepted Answer

GC-MS/MS separates volatile compounds via Gas Chromatography (GC). These enter the first mass spectrometer (MS1) for ionization and mass-to-charge ratio measurement. Specific ions from MS1 are fragmented. The fragments are then analyzed by a second mass spectrometer (MS2). This tandem approach enhances specificity and reduces interference.

Question 5

What is the Application of GC-MS/MS?

Accepted Answer

Clinically, GC-MS/MS profiles steroid hormones in urine, serum, or saliva. It confirms hormone imbalance diagnoses, monitors therapeutic interventions, and assesses metabolic health. Valuable in pediatric endocrinology for newborn screening and forensic toxicology, it assists clinicians in precise diagnostic and treatment decisions.

Question 6

What is the Metric of GC-MS/MS?

Accepted Answer

Results from GC-MS/MS are reported as precise concentrations of specific analytes (e.g., ng/mL, µg/dL, nmol/L). These quantitative measurements are compared against established reference ranges, determined by age, sex, and clinical context. Metabolite presence or absence and their ratios serve as crucial diagnostic indicators.

Question 7

What is the Risk of GC-MS/MS?

Accepted Answer

GC-MS/MS is an analytical technique, posing no direct physical risk. Misinterpretation or misuse of results carries clinical risks. Inaccurate sample collection or handling compromises data integrity. Misdiagnosis or inappropriate treatment may occur if complex data is not interpreted by experienced professionals. Over-reliance on a single test result without the full clinical picture is detrimental.

GC-MS/MS

Meaning

Context

Significance

Mechanism

Application

Metric

Risk

How Do Customs Agencies Verify the Authenticity of Imported Hormonal Therapies?