Question 1

What is the Meaning of Collision-Induced Dissociation?

Accepted Answer

Collision-Induced Dissociation (CID) is a mass spectrometry technique where selected gas-phase ions collide with neutral gas molecules. This energy transfer fragments precursor ions into smaller, characteristic product ions. CID's core purpose is to break down complex molecules into identifiable components, aiding structural elucidation in analytical contexts.

Question 2

What is the Context of Collision-Induced Dissociation?

Accepted Answer

CID is an indispensable analytical tool for studying biological systems, especially in proteomics and metabolomics. It's crucial for advanced platforms characterizing and quantifying endogenous biomolecules like hormones, peptides, and their metabolites in physiological samples, contributing to understanding hormonal health.

Question 3

What is the Significance of Collision-Induced Dissociation?

Accepted Answer

The clinical importance of CID is substantial, offering precision in identifying and quantifying biomolecules critical for patient care. It influences diagnostic accuracy in endocrinology by enabling specific hormone level measurements and disease biomarker detection, guiding personalized therapeutic strategies.

Question 4

What is the Mechanism of Collision-Induced Dissociation?

Accepted Answer

CID involves isolating precursor ions within a mass spectrometer, accelerating them into a collision cell with inert gas. Upon impact, kinetic energy converts to internal vibrational energy, exceeding bond dissociation energy. This causes predictable molecule fragmentation, yielding product ions whose mass-to-charge ratios provide structural insights.

Question 5

What is the Application of Collision-Induced Dissociation?

Accepted Answer

CID is widely applied in clinical and research laboratories, mainly within tandem mass spectrometry (MS/MS). Clinically, it quantifies steroid hormones, thyroid hormones, and vitamin D metabolites, vital for endocrine diagnosis. It also identifies proteins via peptide fragmentation and supports drug metabolism studies.

Question 6

What is the Metric of Collision-Induced Dissociation?

Accepted Answer

CID outcomes are measured via a product ion mass spectrum, displaying mass-to-charge ratios (m/z) and intensities of fragmented ions. These characteristic patterns serve as molecular fingerprints for unambiguous analyte identification. For quantification, product ion intensity correlates with precursor concentration, providing a reliable numerical metric.

Question 7

What is the Risk of Collision-Induced Dissociation?

Accepted Answer

As an analytical method, improper CID application carries risks impacting clinical decisions. Suboptimal fragmentation from inaccurate collision parameter optimization compromises data reliability. Analytical interferences, like matrix effects, may yield false results if not mitigated. Poorly validated CID data without expert oversight could lead to incorrect diagnoses, necessitating quality control.

Collision-Induced Dissociation

Meaning

Context

Significance

Mechanism

Application

Metric

Risk

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