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Chromatogram

Meaning

A Chromatogram is the visual, time-based graphical output generated by a chromatography instrument following the separation of a complex chemical mixture. This graphic typically displays a series of peaks plotted against a time axis, where each distinct peak corresponds to a specific chemical compound or analyte that has been separated from the original sample. In clinical and research settings, the chromatogram serves as the definitive record of the sample’s chemical composition, enabling both the qualitative identification and quantitative measurement of various compounds like steroid hormones or their metabolites.