Question 1

What is the Meaning of ARA70?

Accepted Answer

ARA70, also known as Nuclear Receptor Coactivator 4 (NCOA4), is a protein crucial for regulating steroid hormone receptor activity, especially the androgen receptor (AR). It functions as a coactivator, enhancing transcriptional response of target genes when androgens bind. This protein influences various physiological processes and disease states involving androgen signaling.

Question 2

What is the Context of ARA70?

Accepted Answer

Within the complex cellular signaling network, ARA70 primarily operates in the cell nucleus where steroid hormone receptors reside. It interacts with the androgen receptor's ligand-binding domain, facilitating connection with basal transcription machinery. This coactivator modulates gene expression in androgen-responsive tissues, impacting prostate development, male fertility, and muscle growth.

Question 3

What is the Significance of ARA70?

Accepted Answer

Proper functioning of ARA70 is clinically important due to its influence on androgen receptor activity. Dysregulation of ARA70 expression or function has been implicated in conditions like prostate cancer, where altered androgen signaling contributes to disease progression and therapeutic resistance. Understanding its role aids in comprehending the molecular basis of androgen-dependent disorders.

Question 4

What is the Mechanism of ARA70?

Accepted Answer

ARA70 exerts effects by directly binding to the androgen receptor in a ligand-dependent manner, particularly when androgens are present. This binding stabilizes the receptor-ligand complex and recruits necessary components of the transcriptional machinery, promoting transcription of androgen-responsive genes. It facilitates chromatin remodeling and enhances gene expression.

Question 5

What is the Application of ARA70?

Accepted Answer

In clinical and research settings, studying ARA70 contributes to developing targeted therapies for androgen-related conditions. Its role in prostate cancer progression makes it a potential therapeutic target, with efforts focused on modulating its interaction with the androgen receptor to inhibit uncontrolled cell proliferation. Its expression levels can also be investigated diagnostically.

Question 6

What is the Metric of ARA70?

Accepted Answer

Measurement of ARA70 typically involves molecular biology techniques like quantitative polymerase chain reaction (qPCR) to assess mRNA levels, or Western blotting and immunohistochemistry to determine protein expression within tissue samples. These methods provide insights into its abundance and localization, which can correlate with disease status or treatment response in research studies.

Question 7

What is the Risk of ARA70?

Accepted Answer

While ARA70 is a naturally occurring protein, its dysregulation can pose clinical risks. Overexpression or altered function of ARA70 can contribute to hormone-dependent cancer progression, particularly in androgen receptor signaling in prostate cancer, potentially leading to therapeutic resistance to anti-androgen treatments. Interventions targeting ARA70 require careful consideration of potential off-target effects.

ARA70

Meaning

Context

Significance

Mechanism

Application

Metric

Risk

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